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Bioss
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Image Search Results
Journal: Oncology reports
Article Title: c-Fos mediates α1, 2-fucosyltransferase 1 and Lewis y expression in response to TGF-β1 in ovarian cancer.
doi: 10.3892/or.2017.6052
Figure Lengend Snippet: Figure 5. TGF-β1 activates c-Fos through the MAPK/AP-1 pathway. Upon TGF-β1 treatment, western blotting was employed to determine c-Jun and c-Fos expression in 3 ovarian cells. (A) SKOV3, (B) CAVO3, (C) ES-2. c-Fos and c-Jun expression is related to TGF-β1 in a concentration manner in ovarian cells pretreated with various concentrations of TGF-β1 (0.2, 1, 2, 5 and 10 ng/ml) for 5 min. (D) Western blot analysis was carried out to determine the expression of c-Fos, Lewis y and the downstream signal elements of the MAPK signaling pathway, JNK, p38 and ERK, and the change in the phosphorylation level of JNK, p38 and ERK. CAVO3 cells were treated with pcDNA-c-Fos, c-Fos siRNA, inhibitor of TGF-β1 receptor (LY2109761) and JNK (SPF600125) upon TGF-β1 stimulation.
Article Snippet: When the cells reached 60% confluence, scramble siRNA, c-Jun siRNA and
Techniques: Western Blot, Expressing, Concentration Assay, Phospho-proteomics
Journal: Oncology reports
Article Title: c-Fos mediates α1, 2-fucosyltransferase 1 and Lewis y expression in response to TGF-β1 in ovarian cancer.
doi: 10.3892/or.2017.6052
Figure Lengend Snippet: Figure 4. TGF-β1 promotes cell proliferation via c-Fos in ovarian cancer cells. (A) Cell viability was detected using MTT cell proliferation assay in CAVO3 cells. CAVO3 cells were transfected with pcDNA3-c-Fos, pcDNA3 or c-Fos siRNA for 24 h, followed by pretreatment with TGF-β1. (B and C) Colony formation assessed cell proliferation ability. The cells and processing were the same as shown in a. The data are expressed as the mean ± SD of 3 independent experiments; *P<0.05, compared with the control.
Article Snippet: When the cells reached 60% confluence, scramble siRNA, c-Jun siRNA and
Techniques: MTT Cell Proliferation, Transfection, Control